Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography. Hypothesis
I think that there will be a high RF value in paper chromatography, if we use water solvent because a solvent that has a strong interaction with a chemical will easily over come attraction from the chemical for the absorbent layer and move that chemical in a given period of time. Materials Food Coloring (solute) Ethanol, Acetone, Tap Water, Distilled Water(solvents) Pencil Ruler Scissors Filter Paper Petri Dish Variables Independent: Type of chromatography solvent Dependent: Retention in paper chromatography Controlled: Type of Chromatography paper
Size of spot Thickness of Chromatography Substance tested for paper chromatography Concentration of the solute Temperature Variables Manipulation Independent: Apply different solvents Dependent: The RF value of the given solvent Controlled: Keep the solute same. Keep the size of the solution the same (one drop) Keep the temperature of the room the same. Diagram Method Step 1 – Prepare Materials and pour solvents into the petri dish. Step 2- Fold the provided chromatography paper into half (make sure it can stand) Step 3- measure 1. cm from one end of the paper then draw a line across. This will be used as a starting point. Step 4- Repeat for 4 chromatography papers for each solvent. Step 5- Place a drop of the solution on the pencil line for all 4 chromatography papers Step 6- Mark the chromatography paper to identify it’s solvent Step 7- Fill the 4 different petri dishes with one of each of the solvents Step 8- Make sure the solvents do not reach the pencil line Step 9- Observe as the chromatogram develops
Step 10- Make sure before you take out the chromatography paper, it is entirely wet by the solvent Step 11- Draw a line along the line up to which the solvent has traveled on each chromatography Step 12- Measure the distance between the two pencil lines on the chromatography paper and record the measurement on the top of the paper. Do this for each paper. Step 13- Measure the RF value by dividing “distance travelled by component” with “distance travelled by solvent Step 14- Record the measurement then tabulate the data. Results Solvent| Distance travelled by component| Distance travelled by solvent| RF Value| Tap Water| 5. | 6. 4| 0. 90| Distilled water| 7. 0| 7. 2| 0. 97| Acetone| 4. 7| 5. 5| 0. 85| Ethanol| 3. 1| 6. 1| 0. 50| Graph Reflection In this experiment, I put two drops on each paper; I also used Tap water and Distilled water to get a more reliable result for the solvent. This helped me get me more accurate result. It was hard to get the RF value as I did not get he exact distances (distance travelled by component and solvent) as I got confused on how to take the reading properly. If I do this experiment again, I will surely ask a teacher on how to do so and to get a better and more accurate result.
Conclusion According to the results of the experiment, my hypothesis was proved to be correct. The data shows that the water solvent results as the highest RF value among all the other solvents. It also shows that the solvent results in a great affect retention of chromatography paper. The different types of solvents affect the retention in paper chromatography. In all the solvents I used, water has the greatest effect in retention, as its RF value being the highest among all. Precaution 1. Careful when handling fragile materials 2. Don’t taste or smell solvents 3. Handle chemicals with care
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